Optimization Kits
Catalog
| Product | Catalogue # | Size | List Price | Link |
|---|---|---|---|---|
| Optimization Kit | PTO16101 | 16 Reactions | $1200 | Order |
| Optimization Kit | PTO32101 | 32 Reactions | $2200 | Order |
ℹ️ Need help choosing? Compare our kits
Safety Data Sheets (SDS)
The following Safety Data Sheets apply to these products:Optimize your Single Cell Experiment
Bulk CUT&Tag Optimization Kits are purpose-built for researchers who want to validate, refine, and troubleshoot their single-cell CUT&Tag workflows before committing valuable samples or sequencing resources. By leveraging a modified protocol in a bulk (multi-cell) setting, these kits let you confirm antibody efficiency, adjust nuclear extraction methods, and fine-tune other critical parameters—ensuring your subsequent single-cell experiments have the highest likelihood of success.
What You Need to Get Started
- Antibody: Validated primary antibody for target of interest
- Sequencing: Illumina-compatible sequencing system
Optimization Kits Ensure High-Quality Data
- Verify Assay Compatibility: Assess whether your chosen tissue types, antibodies, and experimental conditions are suitable for single-cell applications—without wasting costly single-cell reagents.
- Fine-Tune Concentrations & Conditions: Adjust sample volumes, reagent concentrations, and incubation times in large titration experiments (up to 32 reactions), pinpointing the optimal protocol for your needs.
- Troubleshoot Common Setup Issues: Diagnostic guidelines and specialized reagents help you quickly identify and resolve challenges like inconsistent nuclear permeabilization or suboptimal antibody binding.
- Scalable Options: Kits come in 16- or 32-reaction formats, accommodating small pilot studies or extensive optimization series across multiple antibodies or tissue types.
- Bridge Bulk to Single-Cell: Compare bulk CUT&Tag data with your future single-cell results, ensuring consistency and reproducibility in downstream profiling.
Performance & Data
- 📈 Deep Complexity: Ensure your CUT&Tag libraries are complex enough for single-cell sequencing with hundreds of millions of recovered fragments
- 🧬 Versatility: Compatible with fresh, frozen, and cryopreserved samples, supporting histone PTMs, chromatin remodelers, and transcription factors
- 🔍Robustness: Exemplary (>95%) correlation to ChIP and Cut&Run demonstrate high reproducibility, ensuring confidence in results.
Get Started
Whether you’re adapting single-cell assays for new tissues, vetting antibody compatibility, or simply refining your protocol to maximize yield, our Bulk CUT&Tag Optimization Kits provide the testing ground you need. Available in 16- and 32-reaction formats, these kits empower you to:
- Validate: Confirm that your selected histone modifications, transcription factors, or sample conditions are suited for single-cell workflows.
- Optimize: Pinpoint the best concentrations and incubation times, ensuring robust signals and minimal background noise.
- Compare: Align bulk data with future single-cell outcomes, guaranteeing consistent and reproducible results.
Optimization: 16 Reaction Kit. Ideal for smaller pilot studies or targeted validation. The 16-Reaction Optimization Kit gives you enough runway to test a handful of antibodies, tissue types, or protocol tweaks without overcommitting resources. Whether you’re investigating a single novel target or confirming conditions for a new tissue, this kit ensures you can optimize key parameters and confidently transition to single-cell CUT&Tag.
Optimization: 32 Reaction Kit. Designed for labs that need a more extensive optimization series across multiple targets or tissue types. The 32-Reaction Optimization Kit enables larger-scale parameter screening—such as broad antibody titrations or parallel workflows for different sample preparations. By allowing more robust comparisons and thorough troubleshooting, it minimizes uncertainty and speeds up your path to high-quality single-cell epigenetic data.
Bulk & Single-Cell Services
If you’re new to targeted single-cell epigenetics, we also provide combined bulk and single-cell experiments as a service — simply send us your samples, and our team will handle everything from assay setup to data delivery. Contact us today to discover how Single-Cell epigenetics can shed light on the dynamic chromatin landscapes underlying your research questions.