Explore Our Latest Research
Discover groundbreaking advancements in single-cell gene regulation at Epigenome Technologies. Dive into our collection of conference posters showcasing cutting-edge discoveries in biomarker identification, immune cell heterogeneity, and more.
Integrative Single-Cell Epigenetic and Transcriptomic Profiling of Human PBMCs Using Droplet Paired-Tag
We profiled PBMCs from two donors using Cut&Tag and Droplet Paired-Tag, generating over 70,000 single-cell profiles. We identified hundreds of thousands of cis-regulatory elements (cCREs), primarily repressive, active, or bivalent. Most cCREs were shared across immune cell types, with a few hundred defining subtypes. Single-cell data confirmed bivalency at specific loci and showed strong correlation with bulk Cut&Tag. These epigenetic landscapes in brain and PBMCs enhance our understanding of immune cell regulation and heterogeneity.
Download Poster (PDF)
Paired-Tag: Single-Cell Epigenetic Profiling for Biomarker Discovery
Single-cell epigenetic profiling identifies key biomarkers of cellular dysfunction and disease. Our methods simultaneously assess RNA expression and histone modifications in individual brain cells and PBMCs, providing high-quality, reproducible data. We discovered novel cell-type-specific cis-regulatory elements (cCREs) and heritability factors linked to Alzheimer's Disease (AD). Additionally, our approach enables the identification of aging and disease-related biomarkers, offering insights into their regulatory mechanisms.
Download Poster (PDF)
Single-Cell Joint Analysis of Histone Modification State and Gene Expression
Applying Paired-Tag to Alzheimer's post-mortem brain tissue, we aligned with SEA-AD RNA data and identified shared pathologies. AD neurons showed H3K9me3 de-repression and H3K27ac activation of genes essential for neuronal identity and synaptic function, linking inflammation to dysfunction. Our single-cell epigenomic and transcriptomic analysis reveals specific cellular changes and potential therapeutic targets, demonstrating Paired-Tag’s effectiveness in understanding and treating complex diseases.
Download Poster (PDF)